“Single Molecules and 3D Super-Resolution, 3D Tracking, and Cryo-CLEM in Cells”
Professor W. E. Moerner
Departments of Chemistry, and by courtesy, of Applied Physics
Stanford University, Stanford, CA USA 94305
Super-resolution microscopy has opened up a new frontier in which biological structures and behavior can be observed in fixed and live cells with resolutions down to 20-40 nm and below. Examples range from protein superstructures in bacteria to bands in axons to details of the shapes of amyloid fibrils, cell surface sugars, protein superstructures in the primary cilium, and much more. For super-resolution imaging in thick cells, a new tilted light sheet design makes use of PSF engineering to create a simple, useful microscope. Low temperature single-molecule imaging provides much improved localization precision in order to complement cryo-electron tomography studies. Additional methods development research addresses ways to use ideas from machine learning and convolutional neural nets to enhance image processing. Combining super-resolution imaging of a static structure with 3D tracking of other biomolecules provides a powerful view of cellular dynamics.
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